Activity Assay:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Derivative Assay:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Sulforhodamine B Assay:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Control:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Concentration Assay:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Formulation:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Inhibition:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Staining:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Expressing:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Western Blot:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Clonogenic Cell Survival Assay:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Cytometry:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Software:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Comparison:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Cell Culture:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Incubation:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Microscopy:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Immunohistochemistry:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Migration:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
MANN-WHITNEY:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Clone Assay:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
shRNA:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Injection:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
In Vivo Imaging:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Phospho-proteomics:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Quantitative Proteomics:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
Immunoprecipitation:Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.
Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.
Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).
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