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hct116 human colon cancer cells  (ATCC)


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    Structured Review

    ATCC hct116 human colon cancer cells
    Hct116 Human Colon Cancer Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 17934 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+colon+cancer+cells+hct116/HCT+116/pm42103106-102-0-5
    Average 99 stars, based on 17934 article reviews
    hct116 human colon cancer cells - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Activity Assay:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Derivative Assay:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Sulforhodamine B Assay:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Control:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Concentration Assay:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Formulation:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Inhibition:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Staining:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Expressing:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Western Blot:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Clonogenic Cell Survival Assay:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Cytometry:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Software:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Comparison:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Cell Culture:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Incubation:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Microscopy:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Immunohistochemistry:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Migration:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    MANN-WHITNEY:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Clone Assay:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    shRNA:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Injection:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    In Vivo Imaging:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Phospho-proteomics:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Quantitative Proteomics:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).

    Immunoprecipitation:

    Article Title: N -Heterocyclic carbene platinum complexes encapsulated in lipid particles: a novel strategy to target cancer cells and cancer stem cells in glioblastoma
    Article Snippet: Temozolomide (TMZ) is toxic at 50 μM to these cells.Temozolomide (TMZ) is toxic at 50 μM to these cells.. Human colon cancer cells HCT116 were purchased from ATCC®.. Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.Both cell lines are adherent cells and were grown in RPMI-1640 medium (Roswell Park Memorial Institute, Sigma-Aldrich, St. Quentin Fallavier, France) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Brazil), 100 U/mL of penicillin and 0.1 mg/mL of streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France) at 37 °C, 95% humidity and 5% CO2.

    Article Title: Enhanced anticancer activity of N,N-bis(5-ethyl-2-hydroxybenzyl)methylamine (EMD) hydrophobic drug encapsulated in β-cyclodextrin nanosponges
    Article Snippet: Human lung cancer cells A549 and human colon cancer cells HCT116 were obtained from the American Type Culture Collection (Manassas, VA, USA).. A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.A549 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HCT-116 cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS), 1 % GlutaMAX, and 1 % penicillin/streptomycin.

    Article Title: Evaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
    Article Snippet: Cell Culture.Cell Culture.. Human colon cancer cells (HCT116) were obtained from the American Type Culture Collection (Rockville, MD, USA).. *e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).*e cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA).

    Article Title: Paladin, overexpressed in colon cancer, is required for actin polymerisation and liver metastasis dissemination.
    Article Snippet: Human colon cancer cells HCT116 (CCL-247) were acquired from ATCC (Manassas, VA, USA).. Additional cell lines were bought from ECACC (LS174t, Lovo).Additional cell lines were bought from ECACC (LS174t, Lovo).



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